Catalog Number - 62308-10P-2

Hyper Similars C8™ Octyl is a traditional reversed-phase HPLC stationary phase developed for laboratories seeking a high-quality alternative to established C8 methods. As part of the Cogent™ Similars product line, it uses high-purity Type-B spherical silica with a bonded octyl (C8) ligand and endcapping to provide reliable chromatographic performance and excellent method reproducibility. Its surface characteristics are designed to closely match those of legacy Hypersil® and Spherisorb® C8 columns, making it particularly useful for method transfer and replacement applications.

62308-10P-2 is a 100 mm x 2.1 mm ID narrow-bore analytical column packed with 3 µm, 150Å Hyper Similars C8 particles. The 100 mm length provides an excellent balance between analysis speed and chromatographic resolution, while the 2.1 mm ID format reduces solvent consumption and is ideally suited for LC-MS applications. The 3 µm particle size delivers greater efficiency and sharper peak shapes than comparable 5 µm columns, although higher operating pressures should be expected. Optimal flow rates are typically approximately 0.2–0.3 mL/min, and the HPLC or LC-MS system should be capable of delivering precise, stable low-flow performance with appropriately low-volume flow cells and connections.

This USP L7 column is an excellent alternative for methods developed on Thermo Fisher Hypersil™ C8, Spherisorb®, and similar octyl phases. The C8 chemistry is well suited for hydrophobic and neutral compounds analyzed under reversed-phase conditions and is particularly useful when less retention is desired than with a C18 column. The narrow-bore format has become a preferred choice for LC-MS applications due to reduced solvent consumption, enhanced MS compatibility, and improved sensitivity.

Key Benefits

• Type-B spherical silica stationary phase

• Suitable alternative for USP L7 methods

• Comparable selectivity to Hypersil® and Spherisorb® C8 columns

• Narrow-bore format ideal for LC-MS applications

• Balanced resolution and run time with 100 mm length

• Higher efficiency with 3 µm particles

• Reduced solvent consumption

• Less retention than C18 columns

Typical Applications

• USP L7 methods

• LC-MS analysis

• Reversed-phase HPLC

• Pharmaceutical analysis

• Quality control testing

• Long-chain fatty acid analysis

• Method transfer and validation

• Routine analytical separations

Specifications

• Product Type: HPLC Column

• Stationary Phase: Hyper Similars C8 Octyl

• USP Classification: L7

• Particle Size: 3 µm

• Particle Type: Type-B Spherical Silica

• Bonded Phase: C8 (Octyl)

• Endcapped: Yes

• Pore Size: 150Å

• Column Dimensions: 2.1 mm ID x 100 mm

• Length: 100 mm

• Recommended Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Narrow-bore format is ideal for LC-MS methods

• Requires an HPLC or LC-MS system capable of precise low-flow operation

• 3 µm particles provide higher efficiency and sharper peaks

• Higher backpressure than comparable 5 µm columns

• Reduced solvent consumption compared to 4.6 mm ID columns

• Excellent alternative for many USP L7 methods